Endocrine Abstracts

Colocalisation of hormones has been demonstrated for growth hormone (GH) and prolactin in the normal adult human pituitary, and for many other hormones in pituitary adenomas. We have investigated whether colocalisation of GH and other hormones occurs earlier, during onto-genesis. Pituitaries from therapeutically aborted male human fetuses of 15–20 weeks gestational age (with ethical approval) were fixed by immersion in 4% buffered formaldehyde and divided in halves, one o...

Colocalisation of hormones in the adult human pituitary has been demonstrated only for growth hormone (GH) and prolactin (PL) and only for adenomatous tissue. We have investigated whether colocalization occurs earlier in ontogenesis. Pituitaries from male human fetuses of 16–22 weeks gestational age fixed by immersion in 4% buffered formaldehyde and embedded in LR Gold were processed for immunocytochemical double labelling with GH/FSH or GH/LH anti-rat and anti-human prim...